(Pro)insulin associates with Golgi membranes of pancreatic B cells.
نویسندگان
چکیده
منابع مشابه
Preparation of Golgi Membranes
Anisotropy of anionic sites on plasma membranes has been implicated in contact phenomena at cell surfaces (16, 17). Recent evidence indicates that charge anisotropy may be important among intracellular membranes also, since distribution of anionic sites has been related to structural organization of mitochondrial membranes (8). The use of cationic ferritin has been introduced by Danon et al. (2...
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The purpose of these experiments was to determine whether insulin-related peptides, larger than proinsulin, could be detected in pancreatic islet cells. Catfish pancreatic islets were incubated with radiolabeled amino acids. After 15- to 60-min incubation, two acid-alcohol-extractable peptides, larger than proinsulin, were detected which were approximately of Mr = 12,000 and 11,000 (12 K and 11...
متن کاملInsulin, not C-peptide (proinsulin), is present in crinophagic bodies of the pancreatic B-cell
We have obtained evidence by autoradiography and immunocytochemistry that mature secretory granules of the pancreatic B-cell gain access to a lysosomal compartment (multigranular or crinophagic bodies) where the secretory granule content is degraded. Whereas the mature secretory granule content shows both insulin and C-peptide (proinsulin) immunoreactivities, in crinophagic bodies only insulin,...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1984
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.81.21.6743